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kacb
To answer your question, the Virginia Company won and was granted a royal charter from King James I of England to start the colony of Virginia. The Company was given the authority to appoint Council of leaders, Governor, and other officials for in the colony. 
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The answer to the question:

What did the Virginia Company win from King James I to start the colony of Virginia? Is that ;

The Virginia Company won the assembly from King James I in order to start the colony of Virginia.